Application

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Dynamin related protein 1 in human embryonic stem cell H9 cells.

This Anti-Dynamin related protein 1 (Drp1) Antibody is validated for use in WB, IC for the detection of Dynamin related protein 1 (Drp1).

Research Sub CategoryOrganelle & Cell MarkersApoptosis - Additional

General description

Dynamin related protein 1 (Drp1), also known as Dynamin-like protein 1, mediates outter mitochondrial membrane fission in mammilian cells. Like other members of the Dynamin superfamily of GTPases, Drp1 contains a N-terminal tripartate GTP-binding domain and exists as a T-shaped dimer with a head, leg, and stalk. The addition of GTP induces head and stalk rearrangement resulting in membrane constriction. Drp1 has been shown to affect mitochondrial morphology and places a significant role in mitochondrial and peroxisomal fission in mammilian cells. During apoptosis, Drp1 translocates from the cytosol to mitochondria and localizes to potential organelle division sites. Studies show Drp1 inhibition averts cell death, reinforcing to the critical role that mitochondrial fission plays in apoptosis.

Immunogen

KLH-conjugated linear peptide corresponding to the GSK3B interaction domain of mouse Dynamin related protein 1 (Drp1).

Epitope: GSK3B interaction domain

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blot in mouse embryonic stem cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Dynamin related protein 1 (Drp1) on 10 µg of mouse embryonic stem cell lysate.

Specificity

This antibody recognizes Dynamin related protein 1 (Drp1) at the GSK3B interaction domain.

Target description

~80kDa (isoform 2) & ~82 kDa (isoform 1) observed
5 isoforms are produced by alternative splicing: Isoform 1: 80 kDa, Isoform 2: 82 kDa, Isoform 3: 78 kDa, Isoform 4: 69 kDa, and Isoform 5: 24 kDa